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Amersham Pharmacia Biotech Ltd multiphor ii apparatus
Multiphor Ii Apparatus, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiphor+ii+apparatus/multiphor+ii+system/pm38002494-116-6-9
Average 90 stars, based on 1 article reviews
multiphor ii apparatus - by Bioz Stars, 2026-09
90/100 stars

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Polyacrylamide Gel Electrophoresis:

Article Title: The YvrI Alternative ? Factor Is Essential for Acid Stress Induction of Oxalate Decarboxylase in Bacillus subtilis
Article Snippet: .. For preparation of 2D PAGE, 200 μg of the protein extracts were separated using nonlinear immobilized pH gradients in the pH range of 3 to 10 (Amersham Biosciences) and a Multiphor II apparatus (Amersham Pharmacia Biotech) as described previously ( 3 ). .. The resulting 2D gels were fixed in 40% (vol/vol) ethanol and 10% (vol/vol) acidic acid and stained with colloidal Coomassie brilliant blue (Amersham Biosciences).

Article Title: The YvrI Alternative σ Factor Is Essential for Acid Stress Induction of Oxalate Decarboxylase in Bacillus subtilis
Article Snippet: .. For preparation of 2D PAGE, 200 g of the protein extracts were separated using nonlinear immobilized pH gradients in the pH range of 3 to 10 (Amersham Biosciences) and a Multiphor II apparatus (Amersham Pharmacia Biotech) as described previously (3). .. The resulting 2D gels were fixed in 40% (vol/vol) ethanol and 10% (vol/vol) acidic acid and stained with colloidal Coomassie brilliant blue (Amersham Biosciences).

Article Title: Reduction in Membrane Phosphatidylglycerol Content Leads to Daptomycin Resistance in Bacillus subtilis
Article Snippet: .. For two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), 200 g of protein extract was separated using nonlinear immobilized pH gradients (IPGs), in the pH range of 4 to 7 for cytoplasmic proteins and 3 to 10 for extracellular and cell wall-associated proteins (Amersham Biosciences), and a Multiphor II apparatus (Amersham Pharmacia Biotech) as described previously (1). .. The resulting 2D gels were fixed in 40% (vol/vol) ethanol and 10% (vol/vol) acetic acid and stained with colloidal Coomassie brilliant blue (Amersham Biosciences).

Electrofocusing:

Article Title: Calcium-Dependent Interaction of Nitric Oxide Synthase with Cytochrome c Oxidase: Implications for Brain Bioenergetics.
Article Snippet: First dimension: The rehydration buffer containing the denatured proteins was used to rehydrate IEF strips (Amersham) overnight at 20–22 ◦C in an Immobiline DryStrip reswelling tray. .. The IEF was performed using the Multiphor II apparatus (Amersham Pharmacia Biotech, Piscataway, NJ, USA). ..

Electrophoresis:

Article Title: Hypoxic preconditioning protects microvascular endothelial cells against hypoxia/reoxygenation injury by attenuating endoplasmic reticulum stress.
Article Snippet: Endothelial cells (ECs) are directly exposed to hypoxia and contribute to injury during myocardial ischemia/ reperfusion.. Hypoxic preconditioning (HPC) protects ECs against hypoxia injury.. This study aimed to explore whether HPC attenuates hypoxia/reoxygenation (H/R) injury by suppressing excessive endoplasmic reticulum stress (ERS) in cultured microvascular ECs (MVECs) from rat heart.

Article Title: Calreticulin downregulation is associated with FGF-2-induced angiogenesis through calcineurin pathway in ischemic myocardium.
Article Snippet: Fibroblast growth factor 2 (FGF-2) plays an integral role in therapeutic angiogenesis associated with myocardial infarct healing.. Calcium (Ca) is one of the most universal important signaling molecules that affect cell proliferation and angiogenesis.. Calreticulin (CRT), a 46-kd Ca-binding chaperone found mainly in the endoplasmic reticulum, plays an important role in regulating calcium homeostasis.

SDS Page:

Article Title: Hypoxic preconditioning protects microvascular endothelial cells against hypoxia/reoxygenation injury by attenuating endoplasmic reticulum stress.
Article Snippet: Endothelial cells (ECs) are directly exposed to hypoxia and contribute to injury during myocardial ischemia/ reperfusion.. Hypoxic preconditioning (HPC) protects ECs against hypoxia injury.. This study aimed to explore whether HPC attenuates hypoxia/reoxygenation (H/R) injury by suppressing excessive endoplasmic reticulum stress (ERS) in cultured microvascular ECs (MVECs) from rat heart.

Article Title: Calreticulin downregulation is associated with FGF-2-induced angiogenesis through calcineurin pathway in ischemic myocardium.
Article Snippet: Fibroblast growth factor 2 (FGF-2) plays an integral role in therapeutic angiogenesis associated with myocardial infarct healing.. Calcium (Ca) is one of the most universal important signaling molecules that affect cell proliferation and angiogenesis.. Calreticulin (CRT), a 46-kd Ca-binding chaperone found mainly in the endoplasmic reticulum, plays an important role in regulating calcium homeostasis.

Two-Dimensional Gel Electrophoresis:

Article Title: Phenotype Enhancement Screen of a Regulatory spx Mutant Unveils a Role for the ytpQ Gene in the Control of Iron Homeostasis
Article Snippet: .. For two-dimensional gel electrophoresis (2D-PAGE), 200 μg of the protein extracts were separated using the non-linear immobilized pH gradients (IPG) in the pH range 4–7 for cytoplasmic proteins (Amersham Biosciences) and a Multiphor II apparatus (Amersham Pharmacia Biotech) as described previously . .. The resulting 2D gels were fixed in 40% (v/v) ethanol, 10% (v/v) acidic acid and stained with Colloidal Coomassie Brilliant Blue (Amersham Biosciences).



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Specification of the Relevant Instrumental Techniques for LF Characterization

Journal: Journal of Agricultural and Food Chemistry

Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties

doi: 10.1021/acs.jafc.3c06887

Figure Lengend Snippet: Specification of the Relevant Instrumental Techniques for LF Characterization

Article Snippet: , IEF , electromigration , Different pI values determined by IEF also might be a matter of the selected separation conditions, e.g. the type of carrier ampholyte (CA), pI marker, buffer composition, and separation time. The possibility of distinction of several LF fractions is based on their pI values. , Multiphor II apparatus (Amersham, Freiburg, Germany); precast gels Servalyt and Blank precotes (Serva, Germany); gel parameters, 126 × 125 × 0.3 mm; temperature, 5–8 °C; voltage, 2000 V; current, 6 mA; power, 12 W , ( , ) .

Techniques: Isolation, Glycoproteomics, Staining, Mass Spectrometry, Concentration Assay, Spectrophotometry, Clinical Proteomics, Zeta Potential Analyzer, Marker, Spectroscopy, Software, Raman Spectroscopy, Functional Assay, Fluorescence, Sequencing, Single Particle, Microscopy, Chromatography, Solvent, Evaporation, Differential Scanning Calorimetry, Injection